Cyclin D1 anticorps

N° du produit ABIN119000

L’anticorps Souris Monoclonal anti-Cyclin D1 a été validé pour WB, FACS, IP, IHC (p), IHC (fro) et EIA. Il convient pour détecter Cyclin D1 dans des échantillons de Humain.

Aperçu rapide pour Cyclin D1 anticorps (ABIN119000)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: CD1-1

Détail du produit anti-Cyclin D1 anticorps

Specificité: This antibody recognises the Cyclin D1.

Purification: Affinity Chromatography on Protein A.

Immunogène: Human Cyclin D1. Spleen cells from immunised mice were fused with cells of the mouse Sp-2 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Membrane permeabilisation is required for this application. ELISA. Immunoprecipitation. Western Blot: 1/500. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000 - 1/10000, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Small intestine, colon.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1

Sujet: Cyclin D1 a 36kD nuclear protein. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy, and over expression has been linked to poor prognosis. During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1

ID gène: 595

NCBI Accession: NP_444284

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling